Polynucleotide kinase function in genetic engineering

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Polynucleotide kinase as a potential target for enhancing cytotoxicity ...Schematic representation of DNA strand breaks induced by topoisomerase inhibitors and the role of PNK in the pathways responsible for their repair. Topo I ... tw | twPhosphatase Component of T4 Polynucleotide Kinase ... - NCBIAmino acids required for catalysis of the 5′ kinase reaction map to the N-terminal half, whereas residues essential for the 3′ phosphatase function cluster in ... tw | twT4 Polynucleotide Kinase | TOYOBO Biotech support DepartmentPRODUCTS | Modifying Enzyme | T4 Polynucleotide Kinase | The category leader, ... to society in the environment, healthcare, and high-function product fields. twT4 Polynucleotide Kinase | NEBcoli strain that carries the cloned T4 Polynucleotide Kinase gene. It is purified by a modification of the method of Richardson (1). Reagents Supplied. twGenetic Screening for Synthetic Lethal Partners of Polynucleotide2012年11月15日 · Polynucleotide kinase/phosphatase (PNKP) is a bifunctional enzyme whose role is to process these termini during SSBR and DSBR by catalyzing ... tw | twT4 Polynucleotide Kinase - Promega CorporationQC Tests: Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay. Unit Definition: One unit is defined as the amount of enzyme required to ... | XRCC1 Stimulates Human Polynucleotide Kinase Activity at ...XRCC1 protein is required for DNA single-strand break repair and genetic stability but its biochemical role is unknown. Here, we report that XRCC1 interacts ...A modified exponential amplification reaction (EXPAR) - RSC ...This method was also successfully applied in the screening of PNK inhibitors and analysis ... ratio for ultrasensitive detection of polynucleotide kinase†.T4 Polynucleotide Kinase (10 U/µL) - Thermo Fisher ScientificThermo Scientific T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded ... twDual Modes of Interaction between XRCC4 and Polynucleotide ...Phosphorylation of XRCC4 by protein kinase CK2 promotes a high affinity ... domain of the end-processing enzyme polynucleotide kinase/phosphatase (PNKP).